e coli and p aeruginosa

e coli and p aeruginosa

Scientists have constructed a strain of engineered E. coli TOP10 that introduces the PLasI promoter induced by acyl-homoserine lactones (AHLs) of Pseudomonas aeruginosa, which drives the expression of gene circuits. In this system, the constitutive tetR promoter initiates transcription, and the produced transcription factor LasR binds to AHL 3OC12HSL, activating the kill and lysis switch, leading to the production of Pyocin S5 protein and E7 lysis protein in the E. coli host. The E7 lysis protein is responsible for breaking down the E. coli host, releasing the Pyocin S5 to kill P. aeruginosa specifically. 

e coli and p aeruginosa