Immortal Cell lines

Human primary cells come from human donors, donated organs, surgical specimens, post-mortem donors, etc. The source limitation is always there while working with primary cell cultures and therefore major planning is crucial while handling primary cells in case of experiments. Also, primary cell cultures derived from explants often do not make homogeneous cultures and purification of specific cell types are required. Primary cells also require additional nutrition and growth factors as they are more sensitive than cell lines. Read here for primary cell culture applications.