Oligonucleotide Synthesis & Purification
Oligonucleotide synthesis is the process of chemically creating short DNA or RNA sequences in the laboratory. It involves the step-by-step assembly of nucleotides to form the desired sequence. Once the synthesis is complete, purification methods are employed to isolate and obtain pure oligonucleotides for various applications, such as research, diagnostics, and therapeutic development.
Types of Oligonucleotide Synthesis & Purification
Chromatography Column
A compact structure and simple operation and maintenance characterize the Hanbon chromatographic column.
Chromatography System
Hanbon’s chromatography system offers the Bio-Lab® laboratory chromatography system, the Bio-Pro® pilot&process chromatography system, among other options, and provides dependable performance and customizable design.
Buffer Dilution/Conditioning System
It addresses the need for complex buffer solutions to be precise and repeatable, minimizes the number of storage tanks, frees up labor and material resources for dispensing, and lowers the cost of production.
Filtration System
Hanbon filtration system is suitable for biopharmaceutical customers with different scales and process routes, including Bio-TFF tangential flow filtration System, Depth filtration system, Membrane cassette holders, etc.
Oligo Synthesizer
Hanbon Sci&Tech independently invented the Bio-Oligo synthesizer, an efficient, quick, and reliable tool for oligonucleotide synthesis.
Preparative Liquid Chromatography System
The separation and purification of pharmaceutical intermediates, biopharmaceuticals, synthetic medications, natural products, fine chemicals, food additives, and other sectors make substantial use of the Hanbon preparative liquid chromatography system.
Chromatographic Column
Hanbon medium and high pressure chromatography columns include laboratory and DAC (dynamic axial compression) columns. Hanbon, which also developed the DAC® (dynamic axial compression) column in 2005, has built the largest production and assembly plant for dynamic axial compression columns.
How Is The Purity Of Synthesized Oligonucleotides Ensured?
The purity of synthesized oligonucleotides is typically assessed using techniques such as high-performance liquid chromatography (HPLC) or capillary electrophoresis (CE). These methods can separate and quantify impurities such as incomplete sequences, truncated products, and deletions. Analytical techniques like mass spectrometry can also be used to confirm the identity and molecular weight of the synthesized oligonucleotides.
